Journal: PLOS Genetics

Article Title: Wild-type bone marrow cells repopulate tissue resident macrophages and reverse the impacts of homozygous CSF1R mutation

doi: 10.1371/journal.pgen.1011525

Figure Lengend Snippet: Antibodies and Reagents.

Article Snippet: , IBA1 , 1:1000 , 120 min , Novachem , 019-19741.

Techniques: Concentration Assay, Incubation, Flow Cytometry, Sequencing

Journal: Development (Cambridge, England)

Article Title: Retrovirus-derived RTL5 and RTL6 genes are novel constituents of the innate immune system in the eutherian brain

doi: 10.1242/dev.200976

Figure Lengend Snippet: RTL6 expression in microglia. (A) Venus-positive cells in the hypothalamus (top), olfactory bulb (middle) and enlarged images of the olfactory bulb region (bottom). Ctx, cerebral cortex; Ob, olfactory bulb. (B) Iba1 staining of the Venus-positive cells in the hypothalamus (top and middle) and enlarged images of the Venus-positive cells (bottom). (C) Isolated microglial cells from the P1 neonatal brain. Left: a population of microglia, Iba1 staining (top) and RTL6 expression (bottom). Right: enlarged pictures of microglia showing RTL6 dots and granules in their cytoplasm.

Article Snippet: They were next stained with DAPI (250 ng/ml) for 10 min at room temperature and washed three times with PBS for 5 min. After carrying out the blocking reaction using PBS containing 5% normal goat serum and 1% BSA at room temperature for 30 min, the sections were first incubated with an anti-Iba1 rabbit antibody (MBL, D513-A48, 1/1000 dilution) or anti-TMEM119 rabbit antibody (Abcam, ab209064, 1/1000 dilution).

Techniques: Expressing, Staining, Isolation

Journal: Frontiers in Neuroscience

Article Title: Effects and mechanism of myeloperoxidase on microglia in the early stage of intracerebral hemorrhage

doi: 10.3389/fnins.2022.1046244

Figure Lengend Snippet: Microglia and MPO were activated following ICH. (A) Immunohistochemistry of the PBS and ICH groups at 6, 12, and 24 h post-injury. The labeled molecule was iba1 (10×, 20×); (B) statistics of iba1 positive expression (** P < 0.01, n = 6 in each group); (C) immunohistochemistry of iba1 in the PBS and ICH groups at 24 h post-injury (10×, 40×); (D) immunofluorescence of MPO in the PBS and ICH groups at 6, 12, and 24 h post-injury (10×, 20×); (E) immunohistochemistry of MPO in the PBS and ICH groups at 6 h post-injury (10×, 40×); (F) statistics of MPO molecular expression (** P < 0.01, *** P < 0.001, n = 6 in each group).

Article Snippet: The antibodies were Rabbit anti iba1 (Solarbio Life Sciences, Beijing, China, 1:600), Goat anti Rabbit (Solarbio, 1:300), and streptavidin–horseradish peroxidase conjugate (STR-HRP).

Techniques: Immunohistochemistry, Labeling, Expressing, Immunofluorescence