Journal: Development (Cambridge, England)

Article Title: Retrovirus-derived RTL5 and RTL6 genes are novel constituents of the innate immune system in the eutherian brain

doi: 10.1242/dev.200976

Figure Lengend Snippet: RTL6 expression in microglia. (A) Venus-positive cells in the hypothalamus (top), olfactory bulb (middle) and enlarged images of the olfactory bulb region (bottom). Ctx, cerebral cortex; Ob, olfactory bulb. (B) Iba1 staining of the Venus-positive cells in the hypothalamus (top and middle) and enlarged images of the Venus-positive cells (bottom). (C) Isolated microglial cells from the P1 neonatal brain. Left: a population of microglia, Iba1 staining (top) and RTL6 expression (bottom). Right: enlarged pictures of microglia showing RTL6 dots and granules in their cytoplasm.

Article Snippet: They were next stained with DAPI (250 ng/ml) for 10 min at room temperature and washed three times with PBS for 5 min. After carrying out the blocking reaction using PBS containing 5% normal goat serum and 1% BSA at room temperature for 30 min, the sections were first incubated with an anti-Iba1 rabbit antibody (MBL, D513-A48, 1/1000 dilution) or anti-TMEM119 rabbit antibody (Abcam, ab209064, 1/1000 dilution).

Techniques: Expressing, Staining, Isolation